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KMID : 0363620080290040146
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Journal of Korean Oriental Medicine
2008 Volume.29 No. 4 p.146 ~ p.160
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Fructus Liquidambaris Contributes to the Chemotaxis of Eosinophils and Secretion of Cytokines in A549 Human Epithelial Cells
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Ryu Han-Chon
Rhee Hyung-Koo
Jung Doo-Young
Jung Sung-Ki
Lim Sabina
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Abstract
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Objectives: The present study was designated to evaluate the direct effects of Fructus Liquidambaris on suppression of eosinophil activity and on suppression of chemokines such as eotaxin, IL-8, ICAM-1, and VCAM-1, in vitro.
Methods: A549 cells were stimulated with TNF- (100 ng/ml), IL-4 (100 ng/ml) or IL-(10 ng/ml) to induce chemokines and adhesion molecules involved in eosinophil chemotaxis. Then after treatment of Fructus Liquidambaris, inhibition effect assay such as ELISA, real-time RT-PCR, and chemotaxis assay was performed.
Results: Eotaxin level was suppressed in both protein secretion and mRNA expression. Eosinophil recruitment to lung epithelial cells was also reduced by Fructus Liquidambaris, implying the role of eotaxin in eosinophil recruitment. In addition, expression of IL-8 was also suppressed by Fructus Liquidambaris (p<0.05). However, expression of adhesion molecules (ICAM-1, VCAM-1) related to eosinophil was not affected. The eosinophil migration was inhibited at high concentration of Fructus Liquidambaris (p<0.05).
Conclusion: These results suggest that Fructus Liquidambaris may regulate a common signaling pathway of eotaxin and IL-8. FS might be of therapeutic value in diseases such as asthma.
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KEYWORD
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Fructus Liquidambaris, eosinophil, eotaxin, asthma, chemokine
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